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Part:BBa_K5152011:Design

Designed by: Paul Lau   Group: iGEM24_HongKong-JSS   (2024-09-26)


3' 20 nt overlapping region for pUC19 EcoRI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The manufacturer reported that a 20 nt overlap provides the highest assembly efficiency.


Source

pUC 19 sequence was obtained from Addgene.

References